10N-Nonyl acridine orange interacts with cardiolipin and allows the quantification of this phospholipid in isolated mitochondria
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منابع مشابه
Visualization of phospholipid domains in Escherichia coli by using the cardiolipin-specific fluorescent dye 10-N-nonyl acridine orange.
Cardiolipin (CL)-specific fluorescent dye 10-N-nonyl-acridine orange (NAO) was used to visualize CL distribution in Escherichia coli cells of different phospholipid compositions. In a filamentous mutant containing only anionic phospholipids, green fluorescent spots were observed along the filaments at approximately regular intervals. Three-dimensional image reconstruction obtained by optical se...
متن کاملUse of the Xuorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on diVerent experimental models
The Xuorescent dye 10-N-nonyl acridine orange (NAO) is extensively used for location and quantitative assays of cardiolipin in living cells on the assumption of its high speciWcity for cardiolipin; however, the limits and the mechanism of this speciWcity are not clear. Moreover, whether factors such as the membrane potential in mitochondria may limit the consistency of the results obtained by t...
متن کاملInhibition of cytochrome c release by 10-N-nonyl acridine orange, a cardiolipin-specific dye, during myocardial ischemia-reperfusion in the rat.
The release of cytochrome c from the mitochondria to the cytosol is a critical step for downstream caspase-mediated apoptotic signal transduction in ischemia-reperfusion (I/R)-induced myocardial tissue injury. 10-N-nonyl acridine orange (NAO), a cardiolipin-specific dye, has been shown to inhibit Bid-mediated cytochrome c release from isolated mitochondria in vitro; however, the possible protec...
متن کاملInhibition of cytochrome c release by 10 - N - nonyl acridine orange , a 1 cardiolipin - specific dye , during myocardial ischemia / reperfusion in the rat 2 3
26 Release of cytochrome c from mitochondria to the cytosol is a critical step for 27 downstream caspase-mediated apoptotic signal transduction in ischemia/reperfusion 28 (I/R)-induced myocardial tissue injury. 10-N-nonyl acridine orange (NAO), a 29 cardiolipin-specific dye, has been shown to inhibit Bid-mediated cytochrome c release 30 from isolated mitochondria in vitro; however, the possible...
متن کاملSuperior fluorescent probe for detection of cardiolipin.
Cardiolipin (CL) is a unique phospholipid found in mitochondrial inner membrane. It is a key component for mitochondrial function in both respiration and apoptosis. The level of CL is an important parameter for investigating these intracellular events and is a critical indicator of a number of diseases associated with mitochondrial respiratory functions. 10-Nonyl acridine orange (NAO) is the on...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1992
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1992.tb17285.x